Morphological alterations in sessile cells of Listeria monocytogenes after treatment with Cymbopogon sp. essential oils

Autores

  • Maíra Maciel Mattos de Oliveira Instituto Federal de Educação, Ciência e Tecnologia
  • Danilo Florisvaldo Brugnera Universidade Federal de Mato Grosso
  • Eduardo Alves Universidade Federal de Lavras
  • Maria das Graças Cardoso Universidade Federal de Lavras
  • Roberta Hilsdorf Piccoli Universidade Federal de Lavras

Resumo

Abstract: Listeria monocytogenes is a pathogenic bacterium capable of forming biofilms on the surfaces of equipment and utensils, contaminating food products. Essential oils have been used as new alternatives to sanitizing solutions elaboration. The aim of this study was to evaluate, by Scanning Electron Microscopy (SEM), the structural effect of Cymbopogon citratus (D.C.) Stapf. (lemongrass) and Cymbopogon nardus (L.) Rendle (citronella) essential oils, applied isolated and in combinated, against L. monocytogenes sessile
cells. The effect was assessed at different stages of biofilm formation on stainless steel surface (3 and 240 hours). For biofilm formation under stainless steel coupons, Tryptic Soy Broth (TSB) was used as substrate and the incubation was performed at 37 °C under agitation at 50 rpm. Sanitizing solutions based on essential oils and a control sanitizing solution were tested at two contact durations (15 and 60 minutes). Then, stainless steel coupons were analyzed by SEM. Several structural alterations were observed in the bacterial
cells after the treatment with essential oil solutions: wrinkled surface aspects, acquisition of a curved appearance, reduction of intracellular content and occurrence of long cells. In contrast,scanning electron micrographs of the sessile cells that had been treated with the control sanitizing solution showed no alteration on the bacterial surface. C. citratus and C. nardus essential oils, isolated and in combination, caused morphological alterations in L. monocytogenes sessile cells. SEM is a valid methodology to evaluate the essential oils structural action against biofilm bacterial cells.

Key words: Cymbopogon nardus, natural sanitizers, structural alterations

Downloads

Não há dados estatísticos.

Referências

AL-REZA, S. M. et al. Potential roles of essential

oil and organic extracts of Zizyphus jujube in

inhibiting food-borne pathogens. Food

Chemistry, v. 119, n. 3, p. 981–986, 2010.

BAJPAI, V. K. et al. Chemical composition,

antibacterial and antioxidant activities of leaf

essential oil and extracts of Metasequioa

glyptostroboides Miki ex Hu. Food and

Chemical Toxicology, v. 47, n. 8, p. 1876–1883,

BAKKALI, F. et al. Biological effects of essential

oils: a review. Food and Chemical Toxicology,

v. 46, n. 2, p. 446-475, 2008.

CAIXETA, D. S. et al. Chemical sanitizers to

control biofilms formed by two Pseudomonas

species on stainless steel surface. Ciência e

Tecnologia de Alimentos, v. 32, n. 1, p. 142-

, 2012.

CARSON, C. F.; MEE, B. J.; RILEY, T. V.

Mechanism of action of Melaleuca alternifolia (tea

tree) oil on Staphylococcus aureus determined by

time-kill, lysis, leakage, and salt tolerance assays

and electron microscopy. Antimicrobial Agents

and Chemotherapy, v. 46, n. 6, p. 1914–1920,

CHORIANOPOULOS, N. G. et al. Disinfectant test

against monoculture and mixed-culture biofilms

composed of technological, spoilage and

pathogenic bacteria: bactericidal effect of

essential oil and hydrosol of Satureja thymbra and comparison with standard acid–base sanitizers.

Journal of Applied Microbiology, v. 104, n. 6, p.

-1696, 2008.

GANDHI, M.; CHIKINDAS, M. L. Listeria: a

foodborne pathogen that knows how to survive.

International Journal of Food Microbiology, v.

, n. 1, p. 1-15, 2007.

KWIECIŃSKI, J.; EICK, S.; WÓJCIK, K. Effects of

tea tree (Melaleuca alternifolia) oil on

Staphylococcus aureus in biofilms and stationary

growth phase. International Journal of

Antimicrobial Agents, v. 33, n. 4, p. 343–347,

KWON, J. A.; YU, C. B.; PARK, H. D.

Bacteriocidal effects and inhibition of cell

separation of cinnamic aldehyde on Bacillus

cereus. Letters in Applied Microbiology, v. 37,

n. 1, p. 61–65, 2003.

MARQUES, S. C. et al. Formation of biofilms by

Staphylococcus aureus on stainless steel and

glass surfaces and its resistance to some selected

chemical sanitizers. Brazilian Journal of

Microbiology, v. 38, n. 3, p. 538-543, 2007.

MILLEZI, A. F. et al. Reduction of Aeromonas

hidrophyla biofilm on stainless stell surface by

essential oils. Brazilian Journal of

Microbiology, v. 44, n.1, p. 73-80, 2013.

OGUNLANA, E. O. et al. Effects of lemongrass oil

on the morphological characteristics and

peptidoglycan synthesis of Escherichia coli cells.

Microbios, v. 50, n. 202, p. 43-59, 1987.

OLIVEIRA, M. M. M. et al. Biofilm formation by

Listeria monocytogenes on stainless steel surface

and biotransfer potential. Brazilian Journal of

Microbiology, v. 41, n. 1, p. 97-106, 2010a.

OLIVEIRA, M. M. M. et al. Disinfectant action of

Cymbopogon sp. essential oils in different phases

of biofilm formation by Listeria monocytogenes on

stainless steel surface. Food Control, v. 21, n. 4,

p. 549-553, 2010b.

OLIVEIRA, M. M. M. et al. Rendimento,

composição química e atividade antilisterial de

óleos essenciais de espécies de Cymbopogon.

Revista Brasileira de Plantas Medicinais, v. 13,

n. 1, p. 8-16, 2011.

SIKKEMA, J.; BONT, J. A. M.; POOLMAN, B.

Mechanisms of membrane toxicity of

hydrocarbons. Microbiological Reviews, v. 59,

n. 2, p. 201-222, 1995.

TEIXEIRA, M. L. et al. Citrumelo Swingle:

Caracterização química, atividade antioxidante e

antifúngica dos óleos essenciais das cascas

frescas e secas. Magistra, v. 24, n. 3, p. 194-203,

VIEIRA, S. S. et al. Composição química e

atividade fungitóxica do óleo essencial de

Ageratum conyzoides L. (Mentrasto). Magistra, v.

, n. 1, p. 55-62, 2012.

Downloads

Publicado

2017-05-26

Como Citar

Oliveira, M. M. M. de, Brugnera, D. F., Alves, E., Cardoso, M. das G., & Piccoli, R. H. (2017). Morphological alterations in sessile cells of Listeria monocytogenes after treatment with Cymbopogon sp. essential oils. MAGISTRA, 26(3), 385–392. Recuperado de https://periodicos.ufrb.edu.br/index.php/magistra/article/view/3993

Edição

v. 26 n. 3 (2014)

Seção

Artigo Científico

Licença

Autores que publicam nesta revista concordam com os seguintes termos:

    1. Autores mantém os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons Attribution que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
    2. Autores têm autorização para assumir contratos adicionais separadamente, para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.