Correlation between sperm motility and hypoosmotic swelling test on cryopreserved goat semen

Autores/as

  • Jurandy Mauro Penitente-Filho Universidade Federal de Viçosa
  • Júlio César Oliveira Dias Universidade Federal de Viçosa
  • Fabrício Albani Oliveira Universidade Federal de Viçosa
  • Camila Oliveira Silveira Universidade Federal de Viçosa
  • Ciro Alexandre Alves Torres Universidade Federal de Viçosa

Resumen

Abstract: The hypoosmotic swelling test (HOST) has been proved to be a good tool for evaluating the membrane integrity of spermatozoa of various domestic animals including goats, cattle, horses, and swine. The aim of this study was to evaluate the correlation between HOST and sperm motility on cryopreserved goat semen. Two adult Parda Alpina breed males were used, totalizing 8 semen collections for each one. Semen was diluted and packaged into 0.25 mL straws. Semen was cooled to 5 °C for 1 hour and pre-freezing was performed in liquid nitrogen vapor for 15 minutes. After this period, the straws were immersed in liquid nitrogen. Samples were thawed in water bath at 37 °C for 30 seconds; then, sperm motility assessment, hypoosmotic swelling test (HOST) and thermoresistance test were performed. Sperm motility and HOST showed high correlation (r = 0.62; P < 0.0001) fitted to a mathematical formula (Sperm Motility = 6.43995 + 1.11884 HOST) by a regression analysis. In conclusion, HOST proved to be a good complementary test to support the analysis of goat sperm motility; however, it should not be used as a unique test for sperm quality assessment.

Keywords: Caprine, Cryopreservation, Statistical assessment

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Citas

Bispo, C.A.S. (2009). Fertility of goat semen cooled or frozen in different concentrations of egg yolk on diluent (96f). Doctoral thesis, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.

Brazilian College of Animal Reproduction. (1998). Manual for andrological assessment and evaluation of animal semen. Belo Horizonte: CBRA.

Bunchaft, G., & Kellner, S.R.O.(1999). Statistics without mystery. Petrópolis: Vozes.

Cabrita, E., Alvarez, R., Anel, E., & Herráez, M.P. (1999). The hypoosmotic swelling test performed with Counter: a method to assay functional integrity of sperm membrane in rainbow trout. Animal Reproduction Science. 55, 279–287.

Callegari-Jacques, S.M. (2003). Biostatistics: principles and applications. Porto Alegre: Artemed.

Castilho, E.F., Guimarães, J.D., Martins, L.F., Pinho, R.O., Guimarães, S.E.F., & Espeschit, C.J.B. (2009). Use of propolis and ascorbic acid on goat semen cryopreservation. Revista Brasileira de Zootecnia, 38, 2335–2345.

Chirineá, V.H., Martins, M.I.M., Souza, F.F., Tebet, J.M., Papa, F.O., & Lopes, M.D. (2006). Morphofunctional characteristics of the cooled and frozen canine semen, using two different diluents Ciência Animal Brasileira, 7, 407–415.

Fonseca, J.F., Torres, C.A.A., Maffili, V.V., Borges, A.M., Santos, A.D.F., Rodrigues, M.T. & Oliveira, R.F.M. (2005). The hypoosmotic swelling test in fresh goat spermatozoa. Animal Reproduction, 2, 139–144.

Fonseca, J.F., Torres, C.A.A., Santos, A.D.F., & Rovay, H. (2001). Hypoosmotic swelling test in goat spermatozoa. Revista Brasileira de Reprodução Animal, 25, 436–438.

Fürst, R., Carvalho, G.R., Fürst, C.O.M., Mafilli, V., Ruas, J.R.M., & Borges, A.M. (2005). Effect of the equine semen cooling on the freezibility. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 57, 599–607.

Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., & Zaneveldi, L.J. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility, 70, 219–228.

Johnson, R.A., & Wichern, D.W. (1988). Applied multivariate statistical analysis. New Jersey: Prentice Hall International.

Kumi-Diaka, J. (1993). Subjecting canine semen to the hypo-osmotic test. Theriogenology. 39, 1279–1289.

Lira, S.A. (2004). Correlation analysis: theoretical approach and construction of coefficients with applications (209f). Doctoral thesis, Federal University of Paraná, Curitiba, Paraná, Brazil.

Lira, S.A., & Neto, A. C. (2006). Correlation coefficient derived from Pearson linear coefficient for ordinal and dichotomic variables. Revista Ciência e Engenharia, 15, 45–53.

Neild, D., Chaves, G., & Flores, M. (1999). Hypoosmotic test in equine spermatozoa. Theriogenology, 51, 721–727.

Revell, S.G., & Mrode, R.A. (1994). An osmotic resistance test for bovine semen. Animal Reproduction Science. 36, 77–86.

Rodrigues, A.S., Ferraz, P.A., Loiola, M.V.G., Bittencourt, R.F., Chalhoub, M., Andrade, B.H.A., Oliveira, S.N., Araujo, E.A.B., & Ribeiro Filho, A.L. (2012). Fractionation of semen doses in FTAI programs in cattle. Magistra, 24 (3), 164-172.

Santos, A.D.F., Torres, C.A.A., Fonseca, J.F., Borges, A.M., Guimarães, J.D., Costa, E.P., & Rovay, H. (2006). Use of complementary tests to evaluate the freezing ability of semen from goats under artificial photoperiod exposure. Revista Brasileira de Zootecnia, 35, 1934–1942.

Siqueira, A.L. (1983). Use of transformation on analysis of variance and analysis of regression. Doctoral thesis, São Paulo University, São Paulo, Brazil.

Statistical Analyses System (2002). SAS/STAT® (Versão 9.0) [Software] Cary: SAS Institute Inc.

Snedecor, G.W., & Cochran, W.G. (1980). Statistical methods. Iowa State University: Ames.

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Publicado

2017-05-22

Cómo citar

Penitente-Filho, J. M., Dias, J. C. O., Oliveira, F. A., Silveira, C. O., & Torres, C. A. A. (2017). Correlation between sperm motility and hypoosmotic swelling test on cryopreserved goat semen. MAGISTRA, 27(3/4), 468–473. Recuperado a partir de https://periodicos.ufrb.edu.br/index.php/magistra/article/view/3940

Número

Vol. 27 Núm. 3/4 (2015)

Sección

Comunicação Científica

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