Pratylenchus spp.: morphological, molecular characterization and population density in banana (Musa spp.) in Pernambuco

Authors

  • Mayara Castro Assunção Universidade Federal Rural de Pernambuco
  • Francisco Jorge Carlos de Souza Junior Universidade Federal Rural de Pernambuco
  • Jaime Corbiniano dos Santos Neto Universidade Federal Rural de Pernambuco
  • Arielena Augusta Rodrigues Mello Universidade Federal Rural de Pernambuco
  • Liany Regina Bezerra de Oliveira Silva Universidade Federal Rural de Pernambuco

Abstract

Abstract: The objective of this study was to determine the identity, morphological characteristics, molecular phylogenetic, and Pratylenchus populations and population density in areas grown with banana trees. Root collections were carried out at each point in banana orchards in Recife. Subsequently, the extractions and morphological and molecular identification of the nematodes were carried out. Two species were identified: P. coffeae and P. brachyurus, with a predominance of P. brachyurus. The occurrence of these species of nematodes, and the prevalence of P. brachyurus inform new data on parasitism Pratylenchus banana, and occasioning severe damage to the crop.

Keywords: Morphometry, Phylogeny, Nematode.

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References

Benson, D.A., et al (2012). GenBank Nucleic Acids Research., 41, 1-7, 10. Doi: 10.1093/nar/gks1195.

Castillo, P., & Vovlas, N. (2007). Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management. In: Hunt, J.D., & Perry, R.N. Nematology monographs and perspectives (p.529). Leiden: Brill Academic Publishers.

Coolen, W.A., & D’Herde, C.J. (1972). A method for the quantitative extraction of nematodes from plant tissue (77p). Ghent: State Agricultural and Entomology Research Station.

Food and Agriculture Organization of the United Nations. (2017). Faostat. Roma: FAO. Recuperado em 20 março, 2020, de http://www.fao.org/faostat/en/#data/QC.

Instituto Brasileiro de Geografia e Estatística. (2018). Pesquisa Agrícola Municipal – SIDRA/PAM. Brasília: IBGE. Recuperado em 20 março, 2020, de https://sidra.ibge.gov.br/tabela/1613.

Jesus, A.M., & Wilcken, S.R.S. (2010). Reprodução de Meloidogyne incognita, M. javanica e Pratylenchus coffeae em diferentes cultivares de bananeira. Nematologia Brasileira, Piracicaba, 34 (4), 3-9.

Jones, J.T., et al. (2013). Top 10 plant‐parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, Oxford, 14 (9), 946-961. DOI: 10.1111/mpp.12057.

Katoh, K., & Toh, H. (2013). MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, Chicago, 30 (4), 772-780. DOI: 10.1093/molbev/mst010.

Kubo, R.K., et al. (2013). Nematoides fitoparasitos da bananeira. In: Nogueira, E. M. C., et al. Bananicultura: manejo fitossanitário e aspectos econômicos e sociais da cultura (pp. 136-163). São Paulo: Instituto Biológico.

Luambano, N. D., et al. (2019). Status of Pratylenchus coffeae in banana-growing areas of Tanzania. Physiological and Molecular Plant Pathology, East Lansing, 105, 102-109. DOI: 10.1016/j.pmpp.2018.08.002.

Miller, M.A., Pfeiffer, W., & Schwartz, T. (2010). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceeding of the Gateway Computing Environments Workshop, New Orleans, 1-8. DOI: 10.1109/GCE.2010.5676129.

Nadler, S., et al. (1999). Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, Leiden, 1 (6), 591-612. DOI: 10.1163/156854199508559.

Powers, T.O., & Harris, T.S. (1993). A polymerase chain reaction method for identification of five major Meloidogyne species. Journal of Nematology, College Park, 25 (1), 1-6.

Sikora, R., Coyne, D., & Quénéhervé, P. (2018). Nematode parasites of bananas and plantains. In: Sikora, R.A., et al. P. Plant Parasitic Nematodes in Subtropical and Tropical Agriculture (pp.617-657). Boston: CABI.

Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, Barcelona, 30 (9), 1312-1313. DOI: 10.1093/bioinformatics/btu033.

Vrain, T.C., Wakarchuk, D.A., Levesque, A.C., & Hamilton, R. I. (1992). Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. Fundamental and Applied Nematology, Montrouge, 5 (6), 563-573

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Published

2020-07-07

How to Cite

Assunção, M. C., Souza Junior, F. J. C. de, Santos Neto, J. C. dos, Mello, A. A. R., & Silva, L. R. B. de O. (2020). Pratylenchus spp.: morphological, molecular characterization and population density in banana (Musa spp.) in Pernambuco. MAGISTRA, 30, 445–449. Retrieved from https://periodicos.ufrb.edu.br/index.php/magistra/article/view/4342

Issue

Vol. 30 (2019)

Section

Comunicação Científica

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